E. Antonio Chiocca, M.D., Ph.D.

Professor and Chairman, Department of Neurological Surgery
Dardinger Family Endowed Chair in Oncological Neurosurgery
Co-director, Dardinger Laboratory for Neuro-oncology and Neurosciences
Co-director, Viral Oncogenesis Program, Comprehensive Cancer Center
                     The Ohio State University Medical Center and Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
Leader, Neuroscience Signature Program
                     The Ohio State University Medical Center and Arthur G. James Cancer Hospital and Richard J. Solove Research Institute

Degree: University of Texas Health Science Center, Houston
Postdoctoral Training: Internship, General Surgery, UTHSC, Houston
                                  Residency, Neurological Surgery, Massachusetts General Hospital, Boston, Massachusetts, Dr. Richard Martuza

Contact Information
N1021 Doan Hall
410 W. 10th Avenue
Columbus, OH  43210
PHONE: (614) 293-9312
FAX: (614) 293-4024
E-MAIL:  EA.Chiocca@osumc.edu

Link to NLM PubMed publications list for E. Antonio Chiocca (last 10 years)

• biologic therapies and applications to disorders of the central nervous system
• functional genomics in brain tumors
• immune responses to gene delivery vehicles

Current Research:

Biologic advances increasingly enable major technological innovations in the treatment of disorders of the central nervous system (CNS). Stem cell biology, interactions between viral and tumor cell life cycles, and definition of gene expression profiles in tumors represent fields of research for possible therapy.

Currently active research areas include:

• engineering more efficient "tumor-killing" vectors
• finding and testing novel prodrug/gene therapy strategies
• defining more selective oncolytic viruses
• characterizing the ability of bone marrow stem cells to “transdifferentiate” into tumor blood vessels or neurons in response to environmental stimuli
• defining the role of the immune response in vector delivery of therapeutic molecules in tumors and in animal models of cerebral ischemia
• developing models of gene transfer to ameliorate cerebrovascular ischemia
• conducting functional genomic studies of neurodegenerative disease using our iBAC system

Techniques:

• Animals:  analysis of transgenic mice; maintenance of knockout mouse lines; small animal surgery
• Cell biology:  cell apoptosis assay; culture of central and peripheral neurons from rats and from wild type and mutant mice; immunocytochemistry; immunoprecipitation; mammalian cell transfection; neuronal cell culture; nuclear and cytoplasmic microinjection; RNA interference; viral infection
• Imaging:  fluorescence and phase contrast confocal microscopy of live and fixed cells; live-animal imaging techniques; microscopic and autoradiographic image processing and analysis and three-dimensional reconstruction and visualization; time-lapse microscopy
• Molecular biology:  cloning viral and nonviral vectors; construction of viral and nonviral expression vectors; DNA-protein interactions; ELISA; flow cytometry; FPLC; gene and microRNA arrays; in situ hybridization; Northern, Southern, and Western blotting; quantitative RT-PCR; RNA isolation; site-specific mutagenesis
• Neurochemistry:  primary cell cultures of CNS neurons, PC12 cells, neuroblastoma, glial, and fibroblast cell lines